Dra. Daphne Pinheiro

Daphne Pinheiro

Professora, doutora em Biologia

Bióloga com PhD em Ciências e ampla experiência em análises clínicas, pesquisa e ensino. Especialista em técnicas laboratoriais avançadas, como PCR, RT-PCR, citometria de fluxo, microscopia, histologia e histoquímica, com sólida atuação em cultura de células e análise de dados. Professora em controle de qualidade e gestão de laboratórios, com expertise em tutoria EAD e uso de plataformas como Moodle. Experiência consolidada em estudos relacionados a terapia celular, fibrose hepática e câncer de mama. Habilidades em redação científica, elaboração de relatórios, trabalho em equipe e gestão de laboratórios, aliadas a um forte compromisso com a excelência e o controle de qualidade.

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Últimas Publicações:

Efeitos do tratamento com meio condicionado de células-tronco mesenquimais em camundongos com fibrose hepática colestática.

Efeito do secretoma de células-tronco derivadas de tecido adiposo cultivadas em duas e três dimensões em camundongos com diabetes tipo 1 induzido por estreptozotocina.

Mecanismos subjacentes à terapia celular na fibrose hepática                                                

Formação Acadêmica.

  • 2010-2014

    Graduação em Ciencias Biologicas

    Análise da expressão de células GFP+ no fígado com fibrose hepática após o transplante de células de medula óssea de camundongos da linhagem C57BL/6 eGFP+.

  • 2014-2016

    Mestrado em Fisiopatologia Clínica e Experimental

    Análise das interações celulares e citocinas envolvidas na regeneração do fígado após o transplante de células de medula óssea em camundongos com fibrose hepática, Ano de Obtenção: 2016

  • 2016-2018

    Especialização em Análises clínicas e patológicas. (Carga Horária: 380h)

    Os escores Child-Pugh e MELD no prognóstico de doenças hepáticas em estágio avançado: Uma revisão sistemática.

  • 2019-2020

    Graduação em Licenciatura para formação de docentes para educação básica em Biologia

    Centro Universitário Augusto Motta, UNISUAM, Brasil.

  • 2016-2021

    Doutorado em Fisiopatologia Clínica e Experimental

    Efeitos do secretoma de células-tronco mesenquimais em modelo experimental de fibrose hepática colestática

Pesquisa.

Terapia Celular e Fibrose Hepática

Pesquisa sobre o uso de células-tronco mesenquimais e seus efeitos terapêuticos na fibrose hepática colestática, com foco em citocinas, perfis celulares e interações celulares.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit.

Check my projects:

Investigation of rabbit’s neuronal reaction What is the main factor of peoples agression?

Análises Clínicas e Biomarcadores

Experiência em técnicas laboratoriais avançadas, como PCR, RT-PCR, citometria de fluxo e histologia, aplicadas no estudo de biomarcadores para doenças hepáticas e câncer de mama.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit.

Check my projects:

Investigation of rabbit’s neuronal reaction What is the main factor of peoples agression?

Pesquisa em Câncer de Mama

Estudos focados nos mecanismos celulares envolvidos na progressão do câncer de mama, com ênfase em cultura de células, histoquímica e análise de dados.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit.

Check my projects:

Investigation of rabbit’s neuronal reaction What is the main factor of peoples agression?

Gestão de Laboratórios e Qualidade

Experiência em controle de qualidade, elaboração de relatórios científicos e gestão eficiente de laboratórios acadêmicos e clínicos.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit.

Check my projects:

Investigation of rabbit’s neuronal reaction What is the main factor of peoples agression?

Tenho grande interesse em pesquisas voltadas para terapia celular, fibrose hepática e câncer de mama, com foco em técnicas laboratoriais avançadas e aplicações clínicas.

Cultura de Células e Terapias Experimentais

Experiência em cultivo tridimensional e bidimensional de células-tronco para avaliação de efeitos terapêuticos em modelos experimentais de diabetes e fibrose hepática.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit.

Check my projects:

Investigation of rabbit’s neuronal reaction What is the main factor of peoples agression?

Publicações.

APX2009 sensitizes hypoxic breast cancer cells to doxorubicin by increasing its accumulation and caspase-3/7-mediated apoptosis

Research, Published: 13 October 2024

The association of targeted therapy with chemotherapy is encouraged to increase the treatment efficiency, especially in hypoxic triple-negative breast cancer. The APE1 redox activity has stood out as a potential tumor target. However, the effect of the association of the APE1 redox inhibitors with doxorubicin in hypoxia still needs to be evidenced. Therefore, our objective was to investigate the effect of the APX2009 (APE1 inhibitor) on the sensitization of breast cancer cells to doxorubicin in normoxia and hypoxia.

Leia o Artigo

Profiles of Expression of SAV1 in Normoxia or Hypoxia Microenviroment are Associated with Breast Cancer Prognosis

Volume 54, Issue 2, February 2023, Pages 79-85

In breast cancer (BC), hypoxia is associated with poor prognosis. Protein Salvador homolog 1 (SAV1) acts as a tumor suppressor and is downregulated in the cancer cells. However, there is limited data on the expression profile of SAV1 and its importance in BC. It has not been studied to evaluate this phenomenon in a hypoxic microenvironment yet.

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Effects of mesenchymal stem cells conditioned medium treatment in mice with cholestatic liver fibrosis

Life Sciences, Volume 281, 15 September 2021, 119768

The synthesis and characterization of a new series of 1,2,3-triazole derivatives containing the 1,3,4-oxadiazole group. The compounds were evaluated for their antimicrobial and anticancer activities. The results indicated that some of these derivatives exhibited significant activity against a variety of pathogenic microorganisms and cancer cells, suggesting their potential as candidates for therapeutic agents.

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Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes

Current Research in Pharmacology and Drug Discovery, Volume 2, 2021, 100069

the synthesis and characterization of a new series of 1,2,3-triazole derivatives containing the 1,3,4-oxadiazole group. The compounds were evaluated for their antimicrobial and anticancer activities. The results indicated that some of these derivatives exhibited significant activity against a variety of pathogenic microorganisms and cancer cells, suggesting their potential as candidates for therapeutic agents.

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Mechanisms Underlying Cell Therapy in Liver Fibrosis: An Overview

Cells 2019, 8(11), 1339; Published: 29 October 2019

The study highlights that mesenchymal stem cells (MSCs) possess anti-inflammatory and antifibrotic properties, which can modulate the hepatic microenvironment and promote tissue regeneration. MSCs exert therapeutic effects through the secretion of cytokines and growth factors that inhibit stellate cell activation and stimulate hepatocyte proliferation.

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Hematopoietic changes in the offspring induced by maternal overweight: Effect on placenta and fetal liver populations

Placenta, Volume 64, April 2018, Pages 7-16

Bone marrow cells (BMC) from obese adult mice display an increased apoptosis rate over proliferation. Hematopoietic stem cells (HSC) form all blood cells and are important BMC used in cell therapy. Because it is known that prenatal development can be affected by adverse metabolic epigenetic programming from the maternal organism, this work aimed to investigate the effects of maternal overweight on placenta and fetal liver hematopoietic niches.

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Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice

PLOS ONE, November 27, 2017

Investigation the migration of bone marrow cells (BMC) to the injured liver after transplantation and their role in liver regeneration. Using a murine model of liver fibrosis induced by bile duct ligation, the researchers transplanted GFP-labeled BMC and observed their localization near regions expressing HGF and SDF-1 in the fibrotic liver. The BMC transplantation restored impaired hepatic cell proliferation in the fibrotic groups.

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Bone marrow mononuclear cell transplantation improves mitochondrial bioenergetics in the liver of cholestatic rats

Experimental Cell Research, Volume 336, Issue 1, 1 August 2015, Pages 15-22

Mitochondrial dysfunction has been associated with liver cholestasis. Toxic bile salt accumulation leads to chronic injury with mitochondrial damage, ROS increase and apoptosis, resulting in liver dysfunction. This study aimed to analyze mitochondrial bioenergetics in rats with hepatic fibrosis induced by bile duct ligation (BDL) after BMMNC transplantation. Livers were collected from normal rats, fibrotic rats after 14 and 21 days of BDL (F14d and F21d) and rats that received BMMNC at 14 days of BDL, analyzed after 7 days. F21d demonstrated increased collagen I content and consequently decrease after BMMNC transplantation. Both F14d and F21d had significantly reduced mitochondrial oxidation capacity and increased mitochondrial uncoupling, which were restored to levels similar to those of normal group after BMMNC transplantation. In addition, F21d had a significantly increase of UCP2, and reduced PGC-1α content. However, after BMMNC transplantation both proteins returned to levels similar to normal group. Moreover, F14d had a significantly increase in 4-HNE content compared to normal group, but after BMMNC transplantation 4-HNE content significantly reduced, suggesting oxidative stress reduction. Therefore, BMMNC transplantation has a positive effect on hepatic mitochondrial bioenergetics of cholestatic rats, increasing oxidative capacity and reducing oxidative stress, which, in turn, contribute to liver function recover.

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Contato.

dra@daphnepinheiro.com.br +55 21 9 8187-0485